论文信息：Guangzheng Sun, Qichao Yang, Ancheng Zhang, Jia Guo, Xinjie Liu, Yang Wang, Qing Ma. Synergistic effect of the combined bio-fungicides ε-poly- L -lysine and chitooligosaccharide in controlling grey mould (Botrytis cinerea) in tomatoes. International Journal of Food Microbiology 276 (2018) 46–53
JCR分区Q1, IF=3.771
论文摘要：The antifungal properties and the induction of resistance by ε-poly- L -lysine (ε-PL) and chitooligosaccharide (COS) were examined to find an alternative to synthetic fungicides currently used in the control of the devas- tating fungal pathogen Botrytis cinerea, the causal agent of grey mould disease of tomatoes. As presented herein, this combined treatment (200mg/L ε-PL +400 mg/L COS) was found to have optimal in vitro antifungal ac- tivities, achieving an inhibition rate of 90.22%. In vivo assays with these combined bio-fungicides, under greenhouse conditions using susceptible tomato plants, demonstrated good protection against severe grey mould. In field tests, the combined bio-fungicides had a control effect of up to 66.67% against tomato grey mould. To elucidate the mechanisms of the combined bio-fungicide-induced resistance in the tomato, plants were subjected to three treatments: 1) inoculation with B. cinerea after spraying with 200 mg/L ε-PL alone, 2) inoculation with the combined bio-fungicides, and 3) inoculation with 400 mg/L COS alone. Compared to the control (sterile water), increases in salicylic acid (SA) and jasmonic acid (JA) levels and increased phenylalanine ammonia lyase (PAL), peroxidase (POD), and superoxide dismutase (SOD) activities were observed. Catalase (CAT) activity and abscisic acid (ABA) and gibberellin (GA) levels decreased, particularly in the combined bio- fungicide-treated plants. Altogether, these findings reveal that the combined bio-fungicides (200mg/L ε- PL +400 mg/L COS) should be an excellent biocontrol agent candidate that combines direct antifungal activity against B. cinerea with plant resistance.
